Journal: Metabolites

Article Title: Regulation of Mitophagy by Low-Intensity Pulsed Ultrasound Attenuates Endothelial Dysfunction

doi: 10.3390/metabo16050329

Figure Lengend Snippet: LIPUS promotes mitophagy in high glucose-stimulated HUVECs. ( A – D ) Representative protein bands and quantitative analysis of p62, LC3-II, and TOM20 protein levels; one-way ANOVA ( n = 3). ( E ) Immunofluorescence co-staining of LC3 (green), TOM20 (red), and DAPI (blue). The scale bar was set to 20 μm ( n = 3). ( F ) 3D surface plot of LC3 fluorescence. ( G ) Fluorescence intensity distribution profiles for LC3 and TOM20. Green represents LC3; red represents TOM20. ( H ) Quantification of LC3-TOM20 co-localization. Co-localization was quantified with the overlap coefficient calculated with ImageJ (Colocalization Finder plugin). A total of 16–21 cells per group were analyzed; one-way ANOVA ( n = 3); ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars represent SD of the mean.

Article Snippet: The primary antibody, Rabbit anti-LC3 antibody (1:200, Abmart, T55992 ) and mouse anti-TOM20 antibody (1:200, Proteintech, Wuhan, China, 666777-1-Ig), diluted in blocking solution, was applied overnight at 4 °C.

Techniques: Immunofluorescence, Staining, Fluorescence

Journal: Redox Biology

Article Title: YY1 nitration participates in DbCM cardiomyocyte lipotoxicity by inhibiting ANXA3 -induced microlipophagy

doi: 10.1016/j.redox.2026.104085

Figure Lengend Snippet: ANXA3 did not regulate myocardial free fatty acid uptake or lipolysis but positively modulated macroautophagy in T2DM mice. (A-C) Relative p62 protein expression level and LC3 II/I ratio in heart tissues of db/m and db/db mice analyzed by Western blot, n = 4. (D-F) Relative p62 protein expression level and LC3 II/I ratio in heart tissues of mice analyzed by Western blot, n = 4. (G-I) Relative p62 protein expression level and LC3 II/I ratio in AC16 cardiomyocytes analyzed by Western blot, n = 6. (J-L) Relative p62 protein expression level and LC3 II/I ratio in AC16 cardiomyocytes analyzed by Western blot, n = 6-8. The data were presented as the mean ± SD. ∗ P < 0.05 versus the db/m group or the db/db + OE-GFP group; ∗∗ P < 0.01 versus the db/m group or the vehicle group or the OE-NC + vehicle group; ∗∗∗ P < 0.001 versus the vehicle group or the OE-NC + vehicle group; # P < 0.05 versus the OE-NC + HGPA group; ### P < 0.001 versus the OE-NC + HGPA group.

Article Snippet: Blocked with 5% (w/v) non-fat-dried milkat room temperature for 1 h. Then the membranes were incubated with the anti-ANXA3 antibody (Proteintech, 11804-1-AP; 1:1000 [v/v]), the anti-YY1 antibody (Proteintech, 22156-1-AP; 1:1000 [v/v]) the anti-PLIN2 antibody (Proteintech, 15294-1-AP; 1:1000 [v/v]), the anti-SQSTM1/p62 antibody (Cell Signaling Technology, 23214; 1:1000 [v/v]), the anti-LC3 antibody (Cell Signaling Technology, 12741; 1:1000 [v/v]), the anti-Rab7 antibody (Proteintech, 55469-1-AP; 1:1000 [v/v])or the rabbit anti-α-Tubulin antibody (ABclonal, AC031; 1:1000 [v/v])overnight at 4 °C.

Techniques: Expressing, Western Blot